哈希水质总氮的测定方法

10071

0.5 –25.0 mg/L N Test N Tube TM ⫼Ѣ∈Ϣ ∈DŽ

Test N Tube TM

1. DBR 200 ⍜㾷 ˈ ⛁㟇105 ćDŽ

ϸϾ2. ⫼ϔϾ ⓣ ˈ

Ԣ䞣⿟Total Nitrogen

Hydroxide ⍜㾷䆩 ㅵЁ ϔ Total Nitrogen

Persulfate ∂䖛⸿䝌Ⲥ䆩 ㉝ DŽ Ⲫ 㶎㒍Ϟ㉬Ϟⱘ䆩 DŽ

ぎⱑ DŽ

ϔϾ䆩 ㅵ3. Ё 2 mLḋ DŽ

䆩 ㅵЁ 2 mL䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

ぎⱑ ˈ 㛑⫼ ϡ ∂ⱘ∈ҷ 䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

⣯4. ϸϾ䆩 ㅵⲪϞⲪ DŽ

⚜ 㟇 30⾦ҹ⏋ DŽ 䖛⸿䝌Ⲥ䆩 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽ

5. 䆩 ㅵ ⍜㾷 ˈⲪϞ6. ⍜㾷 䯈㒧 ˈゟ ⫼ 30 ⛁ⱘ䆩 ㅵҢ⍜㾷

Ё ˈ 䆩 ㅵ Ϟ 㟇 ⏽DŽ

7. 䗝 ⌟䆩⿟ DŽ ✻ⱘ㽕∖ 䗖䜡 䙂 㔽 (䆺㒚ҟ㒡䇋 㾕⫼ ) DŽ 8. 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ Total Nitrogen (TN) A ∂

A 䆩 ㉝ DŽ

Ϟϟ 䆩 ㅵ9. ⲪϞⲪ ˈ

15⾦DŽ

10. Ҿ DŽ䅵 3 䩳DŽ

11. 䅵 䯈㒧 ˈ 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ TN B ∂B 䆩 ㉝ DŽ

12. ⲪϞⲪ ˈϞϟ 䆩 ㅵ15⾦DŽ䆩 ㉝ 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽℸ ⒊⎆ 咘㡆DŽ

Test N Tube TM

13. Ҿ DŽ䅵 2 䩳DŽ

14. 䅵 䯈㒧 ˈ ϸϾTN C ∂C 䆩 ㅵˈ 2 mL ḋ ⍜㾷⎆ ϔϾTN C ∂C 䆩 ㅵЁˈ 2 mLぎⱑ ⍜㾷⎆ ㄀ѠϾTN C ∂C 䆩 ㅵЁDŽ

15. ⲪϞⲪ 䕀䆩 ㅵ10⃵ҹ⏋ DŽ㓧 ⱘ ⱘ 䕀䆩 ㅵDŽ

ℸ 㾺ㅵ䑿⏽ 䆹 ⱘDŽ

16. Ҿ DŽ䅵 5 䩳DŽ

ℸ ⒊⎆ⱘ咘㡆 䆹 ⏅DŽ

17. 䅵 䯈㒧 ˈ ぎⱑ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

18. ϟ“Zero()”䬂䖯㸠Ҿ 䇗䳊DŽ䖭 ⼎˖

0.0 mg/L N

19. 㺙 ḋ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

ҹ䖯㸠 ϔ㒘ⱘ Ͼḋ ⱘ⌟䆩DŽ

20. ϟ“Read()”䬂䇏 ∂ 䞣ˈ㒧 ҹmg/L NЎ ԡDŽ

ℸ 䯈 ҹ⫼ℸぎⱑ ⌟ 㒘ⱘ Ͼḋ DŽ ぎⱑ 䰈 ˈ⏽ ⏽(18 – 25 ぎⱑ 䆩 䭓 ҹ 7 ˈ

ć) DŽ ϔ ˈぎ ⱑ䆩 ⦄ 䞣ⱘⱑ㡆㍂ ⠽ˈ䇋㟡 䖭Ͼ 䞡 䜡 ⱘぎ ⱑ䆩 DŽ

ϟ㸼 Ё ⼎ⱘ ⠽ ⱘ ⌧ ∈ (mg/L)ϟ ⌟䆩ϡѻ⫳ DŽ㛑 ѻ⫳f10%⌟䆩䇃 ⱘ ⠽䋼 㸼ЁDŽ

⌟䆩 ⫼ҹϟ⠽䋼䜡 ⱘ∂ ⒊⎆䖯㸠ⱘˈ 95%ⱘ ⥛DŽ g ∃ 䫉 g ㋴ g ⸿䝌䫉 g ⇼ Э䝌 g 䝟䝌

⫼∃ 䫉 ⚳⺅䝌-PTSA ⱘ ⥛ˈ ⫳⌏∵∈䖯 ∈ǃ ҷ∵∈(D5905 - 96)ⱘASTM ⠽䋼Ёⱘ ⥛䛑ı95%DŽ ѯḋ Ё ⱘ 䞣ϡ ∂ⱘ ⠽Ӯ Ў⍜㗫 ⍜㾷䆩 Ёⱘ䖛⸿䝌Ⲥ㗠䰡Ԣњ⍜㾷ⱘ ⥛DŽ ⶹḋ Ё 䕗催⌧ ⱘ䖭⾡ ⠽ˈ 䆹⿔䞞ḋ ˈ 催⍜㾷 ⥛ 䞡 䖯㸠⌟䆩DŽ

ḋ 䞛䲚 Փ⫼⏙⋕ⱘ⦏⩗ DŽ䞛ḋ ゟ ⱘ㒧 䴴DŽ

䞛ḋ ϡ㛑ゟ 䖯㸠 ⌟䆩ˈ䇋Փ⫼⸿䝌(2mL⌧⸿䝌/ L∈) ḋ ⱘpH 䇗 㟇2 㗙2ҹϟҹ DŽ ḋ 㕂Ѣ4 ć ( 39 ̧ )ⱘ ӊϟ䖯㸠 DŽḋ 䭓 ҹ 28 DŽ

⌟䆩 ˈ䇋 ḋ ⛁㟇 ⏽ˈ⫼5.0 N⇶⇻ 䩴⒊⎆Ё ḋ 䝌 ˈ ḋ ⱘpH 䇗 Ё DŽ ḍ ḋ ԧ⿃ 䞣 ℷ⌟䆩㒧 DŽ

⊩ Ѣ ∂䗮 㛑 䖒 95 ̢ 100%ⱘ ⥛DŽ ⊩⫼ ⇣∂ ⠽䋼⊩䖯㸠 ⹂ Ẕ DŽ

1. 䜡 ҹϟϝϾ⒊⎆ЁⱘϔϾ Ͼ⒊⎆DŽ↣ϔ⾡⒊⎆ⱘ⌧ 䛑Ⳍ Ѣ25 mg/L N ∂ ⒊⎆DŽ⫼䆩 㒘ӊЁⱘ ⾏ ∈ 㗙ϡ ӏԩ ⠽ ∂ⱘ∈䜡 䖭ѯ ⒊⎆DŽ

a. ⿄ 0.3379 g ⱘ 㣃⻎䝌䫉Ammonium p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

b. ⿄ 0.4416 g ⱘ⇼ Э䝌 㣃⻎䝌Glycine p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

c. ⿄ 0.5274 g ⱘ⚳⺅䝌 㣃⻎䝌Nicotinic p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

2. ⫼Ϟ䗄 TM ⒊⎆䖯㸠⌟ DŽ⫼ϟ䴶ⱘ 䅵ㅫ ⥛DŽ 䆺㒚 䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

% ⥛ =

䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

⌟ ⱘ⌧

u 100

25

()

⹂ Ẕ 䳔ⱘ䆩 ϢҾ ˖

⇼∂ ⒊⎆ˈ⌧ Ў1000 mg/L NH3 – N ⫓⫊ TenSette ⿏⎆ 䜡 ⱘ ⏋ 䞣ㄦˈϝϾ

1. 䇏 ⌟䆩㒧 ˈ 㺙 ḋ ⱘ↨㡆ⲓ( ⠽䋼) ⬭ Ҿ ЁDŽ

ˈ ⱘḋ 䇏 ⼎ 乊ッⱘϔ㸠DŽ 䆺㒚 䇋 㾕⫼ DŽ 4. ⌧ Ў1000 mg/L NH3 – Nⱘ⇼∂ ⒊⎆DŽ 5. ⫼TenSette ⿏⎆ ϝϾ ḋDŽ ḋ ϝϾ⏋ 䞣ㄦЁˈ⎆䴶Ϣ10 mL 㒓 唤DŽՓ⫼TenSette ⿏⎆ ϝϾ⏋ 䞣ㄦЁձ⃵ 0.1 mLˈ0.2 mL 0.3 mLⱘ ⠽䋼ˈ⏋ DŽ

6. Ң0.1 mLⱘ ḋ ˈ ✻Ϟ䗄 þ TMձ⃵ ϝϾ ḋ 䖯㸠⌟䆩DŽ “Read()”䬂⹂䅸 ↣ϔϾ ḋ ⱘ⌟䆩 DŽ

7. ⌟䆩䖛⿟㒧 ˈ “Graph()”䬂 ⼎ ḍ 䅵ㅫ ⱘ Շ 㒓ˈ䇈 ⱘ Ϣ DŽ “Ideal Line()”䬂 ⼎ ḋ Ϣ100% ⥛ⱘ“⧚ 㒓 ”П䯈ⱘ ㋏DŽ↣Ͼ ḋ䛑 䆹䖒 㑺100%ⱘ ⥛DŽ

䳔ⱘ䆩 ϢҾ 䆒 ˖

⇼∂ ⒊⎆ˈ⌧ Ў10 mg/L

TM

1. ⫼2mL ⌧ Ў10 mg/Lⱘ⇼∂ ⒊⎆ҷ ḋ ✻Ϟ䗄 Test

䖯㸠⌟䆩DŽ

⒊⎆⌧ 㒓DŽ

⺅ ⱘ䖛⸿䝌Ⲥ⍜㾷䖛⿟ ⱘ∂䛑䕀 Ў⸱䝌ⲤDŽ⍜㾷㒧 ⱘ Ѯ⸿䝌⇶䩴⫼Ѣ 䰸 ㋴㉏⇻ ⠽䋼DŽ ⸱䝌ⲤϢ 㡆䝌 䝌 ⦃ ϟ ⫳ ϔ⾡咘㡆䜡 ⠽DŽ⌟䆩㒧 ⊶䭓Ў410 nmⱘ 㾕 ϟ䇏 ⱘDŽ

10072

2 – 150 mg/L N Test N Tube TM ⫼Ѣ∈Ϣ ∈DŽ

Test N Tube TM

1. DBR 200 ⍜㾷 ˈ ⛁㟇105 ćDŽ

ϸϾ2. ⫼ϔϾ ⓣ ˈ

催䞣⿟Total Nitrogen

Hydroxide ⍜㾷䆩 ㅵЁ ϔ Total Nitrogen

Persulfate ∂䖛⸿䝌Ⲥ䆩 ㉝ DŽ Ⲫ 㶎㒍Ϟ㉬ⱘ䆩 DŽ

ϔϾ䆩 ㅵ3. Ё 0.5 mLḋ DŽ

䆩 ㅵЁ 0.5 mL䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

ぎⱑ ˈ 㛑⫼ ϡ ∂ⱘ∈ҷ 䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

⣯4. ϸϾ䆩 ㅵⲪϞⲪ DŽ

⚜ 㟇 30⾦ҹ⏋ DŽ 䖛⸿䝌Ⲥ䆩 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽ

5. 䆩 ㅵ ⍜㾷 ˈⲪϞ6. ⍜㾷 䯈㒧 ˈゟ ⫼ Ⲫ DŽ ⛁⍜㾷ℷ 30 䩳DŽ ⛁ⱘ䆩 ㅵҢ⍜㾷

Ё ˈ 䆩 ㅵ Ϟ 㟇 ⏽DŽ

7. 䗝 ⌟䆩⿟ DŽ ✻ⱘ㽕∖ 䗖䜡 䙂 㔽 (䆺㒚ҟ㒡䇋 㾕⫼ ) DŽ

8. 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ Total Nitrogen (TN) A ∂A 䆩 ㉝ DŽ

Ϟϟ 䆩 ㅵ9. ⲪϞⲪ ˈ

15⾦DŽ

10. Ҿ DŽ䅵 3 䩳DŽ

11. 䅵 䯈㒧 ˈ 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ TN B ∂B 䆩 ㉝ DŽ

12. ⲪϞⲪ ˈϞϟ 䆩 ㅵ15⾦DŽ䆩 ㉝ 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽℸ ⒊⎆ 咘㡆DŽ

Test N Tube TM

13. Ҿ DŽ䅵 2 䩳DŽ

14. 䅵 䯈㒧 ˈ ϸϾTN C ∂C 䆩 ㅵˈ 2 mL ḋ ⍜㾷⎆ ϔϾTN C ∂C 䆩 ㅵЁˈ 2 mLぎⱑ ⍜㾷⎆ ㄀ѠϾTN C ∂C 䆩 ㅵЁDŽ

15. ⲪϞⲪ 䕀䆩 ㅵ10⃵ҹ⏋ DŽ㓧 ⱘ ⱘ 䕀䆩 ㅵDŽ

ℸ 㾺ㅵ䑿⏽ 䆹 ⱘDŽ

16. Ҿ DŽ䅵 5 䩳DŽ

ℸ ⒊⎆ⱘ咘㡆 䆹 ⏅DŽ

17. 䅵 䯈㒧 ˈ ぎⱑ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

18. ϟ“Zero()”䬂䖯㸠Ҿ 䇗䳊DŽ䖭 ⼎˖

0 mg/L N

19. 㺙 ḋ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

ҹ䖯㸠 ϔ㒘ⱘ Ͼḋ ⱘ⌟䆩DŽ

20. ϟ“Read()”䬂䇏 ∂ 䞣ˈ㒧 ҹmg/L NЎ ԡDŽ

ℸ 䯈 ҹ⫼ℸぎⱑ ⌟ 㒘ⱘ Ͼḋ DŽ ぎⱑ 䰈 ˈ⏽ ⏽(18 – 25 ぎⱑ 䆩 䭓 ҹ 7 ˈ

ć) DŽ ϔ ˈぎ ⱑ䆩 ⦄ 䞣ⱘⱑ㡆㍂ ⠽ˈ䇋㟡 䖭Ͼ 䞡 䜡 ⱘぎ ⱑ䆩 DŽ

ϟ㸼 Ё ⼎ⱘ ⠽ ⱘ ⌧ ∈ (mg/L)ϟ ⌟䆩ϡѻ⫳ DŽ㛑 ѻ⫳f10%⌟䆩䇃 ⱘ ⠽䋼 㸼ЁDŽ

⌟䆩 ⫼ҹϟ⠽䋼䜡 ⱘ∂ ⒊⎆䖯㸠ⱘˈ 95%ⱘ ⥛DŽ g ∃ 䫉 g ㋴ g ⸿䝌䫉 g ⇼ Э䝌 g 䝟䝌

⫼∃ 䫉 ⚳⺅䝌-PTSA ⱘ ⥛ˈ ⫳⌏∵∈䖯 ∈ǃ ҷ∵∈(D5905 - 96)ⱘASTM ⠽䋼Ёⱘ ⥛䛑ı95%DŽ ѯḋ Ё ⱘ 䞣ϡ ∂ⱘ ⠽Ӯ Ў⍜㗫 ⍜㾷䆩 Ёⱘ䖛⸿䝌Ⲥ㗠䰡Ԣњ⍜㾷ⱘ ⥛DŽ ⶹḋ Ё 䕗催⌧ ⱘ䖭⾡ ⠽ˈ 䆹⿔䞞ḋ ˈ 催⍜㾷 ⥛ 䞡 䖯㸠⌟䆩DŽ

ḋ 䞛䲚 Փ⫼⏙⋕ⱘ⦏⩗ DŽ䞛ḋ ゟ ⱘ㒧 䴴DŽ

䞛ḋ ϡ㛑ゟ 䖯㸠 ⌟䆩ˈ䇋Փ⫼⸿䝌(2mL⌧⸿䝌/ L∈) ḋ ⱘpH 䇗 㟇2 㗙2ҹϟҹ DŽ ḋ 㕂Ѣ4 ć ( 39 ̧ )ⱘ ӊϟ䖯㸠 DŽḋ 䭓 ҹ 28 DŽ

⌟䆩 ˈ䇋 ḋ ⛁㟇 ⏽ˈ⫼5.0 N⇶⇻ 䩴⒊⎆Ё ḋ 䝌 ˈ ḋ ⱘpH 䇗 Ё DŽ ḍ ḋ ԧ⿃ 䞣 ℷ⌟䆩㒧 DŽ

⊩ Ѣ ∂䗮 㛑 䖒 95 ̢ 100%ⱘ ⥛DŽ ⊩⫼ ⇣∂ ⠽䋼⊩䖯㸠 ⹂ Ẕ DŽ

1. 䜡 ҹϟϝϾ⒊⎆ЁⱘϔϾ Ͼ⒊⎆DŽ↣ϔ⾡⒊⎆ⱘ⌧ 䛑Ⳍ Ѣ25 mg/L N ∂ ⒊⎆DŽ⫼䆩 㒘ӊЁⱘ ⾏ ∈ 㗙ϡ ӏԩ ⠽ ∂ⱘ∈䜡 䖭ѯ ⒊⎆DŽ

a. ⿄ 1.6208 g ⱘ 㣃⻎䝌䫉Ammonium p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

b. ⿄ 2.1179 g ⱘ⇼ Э䝌 㣃⻎䝌Glycine p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

c. ⿄ 2.5295 g ⱘ⚳⺅䝌 㣃⻎䝌Nicotinic p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

2. ⫼Ϟ䗄 TM ⒊⎆䖯㸠⌟ DŽ⫼ϟ䴶ⱘ 䅵ㅫ ⥛DŽ 䆺㒚 䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

% ⥛ =

䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

⌟ ⱘ⌧

u 100

25(120)

()

⹂ Ẕ 䳔ⱘ䆩 ϢҾ ˖

⇼∂ ⒊⎆ˈ⌧ Ў1000 mg/L NH3 – N ⫓⫊ TenSette ⿏⎆ 䜡 ⱘ ⏋ 䞣ㄦˈϝϾ

1. 䇏 ⌟䆩㒧 ˈ 㺙 ḋ ⱘ↨㡆ⲓ( ⠽䋼) ⬭ Ҿ ЁDŽ

ˈ ⱘḋ 䇏 ⼎ 乊ッⱘϔ㸠DŽ 䆺㒚 䇋 㾕⫼ DŽ 4. ⌧ Ў1000 mg/L NH3 – Nⱘ⇼∂ ⒊⎆DŽ 5. ⫼TenSette ⿏⎆ ϝϾ ḋDŽ ḋ ϝϾ⏋ 䞣ㄦЁˈ⎆䴶Ϣ25 mL 㒓 唤DŽՓ⫼TenSette ⿏⎆ ϝϾ⏋ 䞣ㄦЁձ⃵ 0.1 mLˈ0.2 mL 0.3 mLⱘ ⠽䋼ˈ⏋ DŽ

6. Ң0.1 mLⱘ ḋ ˈ ✻Ϟ䗄 Test TMձ⃵ ϝϾ ḋ 䖯㸠⌟䆩DŽ “Read()”䬂⹂䅸 ↣ϔϾ ḋ ⱘ⌟䆩 DŽ

7. ⌟䆩䖛⿟㒧 ˈ “Graph()”䬂 ⼎ ḍ 䅵ㅫ ⱘ Շ 㒓ˈ䇈 ⱘ Ϣ DŽ “Ideal Line()”䬂 ⼎ ḋ Ϣ100% ⥛ⱘ“⧚ 㒓 ”П䯈ⱘ ㋏DŽ↣Ͼ ḋ䛑 䆹䖒 㑺100%ⱘ ⥛DŽ

䳔ⱘ䆩 ϢҾ 䆒 ˖

⇼∂ ⒊⎆ˈ⌧ Ў100 mg/L

TM

1. ⫼0.5mL ⌧ Ў100 mg/Lⱘ⇼∂ ⒊⎆ҷ ḋ ✻Ϟ䗄 Test þ䖯㸠⌟䆩DŽ

⒊⎆⌧ 㒓DŽ

⺅ ⱘ䖛⸿䝌Ⲥ⍜㾷䖛⿟ ⱘ∂䛑䕀 Ў⸱䝌ⲤDŽ⍜㾷㒧 ⱘ Ѯ⸿䝌⇶䩴⫼Ѣ 䰸 ㋴㉏⇻ ⠽䋼DŽ ⸱䝌ⲤϢ 㡆䝌 䝌 ⦃ ϟ ⫳ ϔ⾡咘㡆䜡 ⠽DŽ⌟䆩㒧 ⊶䭓Ў410 nmⱘ 㾕 ϟ䇏 ⱘDŽ

10071

0.5 –25.0 mg/L N Test N Tube TM ⫼Ѣ∈Ϣ ∈DŽ

Test N Tube TM

1. DBR 200 ⍜㾷 ˈ ⛁㟇105 ćDŽ

ϸϾ2. ⫼ϔϾ ⓣ ˈ

Ԣ䞣⿟Total Nitrogen

Hydroxide ⍜㾷䆩 ㅵЁ ϔ Total Nitrogen

Persulfate ∂䖛⸿䝌Ⲥ䆩 ㉝ DŽ Ⲫ 㶎㒍Ϟ㉬Ϟⱘ䆩 DŽ

ぎⱑ DŽ

ϔϾ䆩 ㅵ3. Ё 2 mLḋ DŽ

䆩 ㅵЁ 2 mL䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

ぎⱑ ˈ 㛑⫼ ϡ ∂ⱘ∈ҷ 䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

⣯4. ϸϾ䆩 ㅵⲪϞⲪ DŽ

⚜ 㟇 30⾦ҹ⏋ DŽ 䖛⸿䝌Ⲥ䆩 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽ

5. 䆩 ㅵ ⍜㾷 ˈⲪϞ6. ⍜㾷 䯈㒧 ˈゟ ⫼ 30 ⛁ⱘ䆩 ㅵҢ⍜㾷

Ё ˈ 䆩 ㅵ Ϟ 㟇 ⏽DŽ

7. 䗝 ⌟䆩⿟ DŽ ✻ⱘ㽕∖ 䗖䜡 䙂 㔽 (䆺㒚ҟ㒡䇋 㾕⫼ ) DŽ 8. 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ Total Nitrogen (TN) A ∂

A 䆩 ㉝ DŽ

Ϟϟ 䆩 ㅵ9. ⲪϞⲪ ˈ

15⾦DŽ

10. Ҿ DŽ䅵 3 䩳DŽ

11. 䅵 䯈㒧 ˈ 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ TN B ∂B 䆩 ㉝ DŽ

12. ⲪϞⲪ ˈϞϟ 䆩 ㅵ15⾦DŽ䆩 ㉝ 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽℸ ⒊⎆ 咘㡆DŽ

Test N Tube TM

13. Ҿ DŽ䅵 2 䩳DŽ

14. 䅵 䯈㒧 ˈ ϸϾTN C ∂C 䆩 ㅵˈ 2 mL ḋ ⍜㾷⎆ ϔϾTN C ∂C 䆩 ㅵЁˈ 2 mLぎⱑ ⍜㾷⎆ ㄀ѠϾTN C ∂C 䆩 ㅵЁDŽ

15. ⲪϞⲪ 䕀䆩 ㅵ10⃵ҹ⏋ DŽ㓧 ⱘ ⱘ 䕀䆩 ㅵDŽ

ℸ 㾺ㅵ䑿⏽ 䆹 ⱘDŽ

16. Ҿ DŽ䅵 5 䩳DŽ

ℸ ⒊⎆ⱘ咘㡆 䆹 ⏅DŽ

17. 䅵 䯈㒧 ˈ ぎⱑ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

18. ϟ“Zero()”䬂䖯㸠Ҿ 䇗䳊DŽ䖭 ⼎˖

0.0 mg/L N

19. 㺙 ḋ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

ҹ䖯㸠 ϔ㒘ⱘ Ͼḋ ⱘ⌟䆩DŽ

20. ϟ“Read()”䬂䇏 ∂ 䞣ˈ㒧 ҹmg/L NЎ ԡDŽ

ℸ 䯈 ҹ⫼ℸぎⱑ ⌟ 㒘ⱘ Ͼḋ DŽ ぎⱑ 䰈 ˈ⏽ ⏽(18 – 25 ぎⱑ 䆩 䭓 ҹ 7 ˈ

ć) DŽ ϔ ˈぎ ⱑ䆩 ⦄ 䞣ⱘⱑ㡆㍂ ⠽ˈ䇋㟡 䖭Ͼ 䞡 䜡 ⱘぎ ⱑ䆩 DŽ

ϟ㸼 Ё ⼎ⱘ ⠽ ⱘ ⌧ ∈ (mg/L)ϟ ⌟䆩ϡѻ⫳ DŽ㛑 ѻ⫳f10%⌟䆩䇃 ⱘ ⠽䋼 㸼ЁDŽ

⌟䆩 ⫼ҹϟ⠽䋼䜡 ⱘ∂ ⒊⎆䖯㸠ⱘˈ 95%ⱘ ⥛DŽ g ∃ 䫉 g ㋴ g ⸿䝌䫉 g ⇼ Э䝌 g 䝟䝌

⫼∃ 䫉 ⚳⺅䝌-PTSA ⱘ ⥛ˈ ⫳⌏∵∈䖯 ∈ǃ ҷ∵∈(D5905 - 96)ⱘASTM ⠽䋼Ёⱘ ⥛䛑ı95%DŽ ѯḋ Ё ⱘ 䞣ϡ ∂ⱘ ⠽Ӯ Ў⍜㗫 ⍜㾷䆩 Ёⱘ䖛⸿䝌Ⲥ㗠䰡Ԣњ⍜㾷ⱘ ⥛DŽ ⶹḋ Ё 䕗催⌧ ⱘ䖭⾡ ⠽ˈ 䆹⿔䞞ḋ ˈ 催⍜㾷 ⥛ 䞡 䖯㸠⌟䆩DŽ

ḋ 䞛䲚 Փ⫼⏙⋕ⱘ⦏⩗ DŽ䞛ḋ ゟ ⱘ㒧 䴴DŽ

䞛ḋ ϡ㛑ゟ 䖯㸠 ⌟䆩ˈ䇋Փ⫼⸿䝌(2mL⌧⸿䝌/ L∈) ḋ ⱘpH 䇗 㟇2 㗙2ҹϟҹ DŽ ḋ 㕂Ѣ4 ć ( 39 ̧ )ⱘ ӊϟ䖯㸠 DŽḋ 䭓 ҹ 28 DŽ

⌟䆩 ˈ䇋 ḋ ⛁㟇 ⏽ˈ⫼5.0 N⇶⇻ 䩴⒊⎆Ё ḋ 䝌 ˈ ḋ ⱘpH 䇗 Ё DŽ ḍ ḋ ԧ⿃ 䞣 ℷ⌟䆩㒧 DŽ

⊩ Ѣ ∂䗮 㛑 䖒 95 ̢ 100%ⱘ ⥛DŽ ⊩⫼ ⇣∂ ⠽䋼⊩䖯㸠 ⹂ Ẕ DŽ

1. 䜡 ҹϟϝϾ⒊⎆ЁⱘϔϾ Ͼ⒊⎆DŽ↣ϔ⾡⒊⎆ⱘ⌧ 䛑Ⳍ Ѣ25 mg/L N ∂ ⒊⎆DŽ⫼䆩 㒘ӊЁⱘ ⾏ ∈ 㗙ϡ ӏԩ ⠽ ∂ⱘ∈䜡 䖭ѯ ⒊⎆DŽ

a. ⿄ 0.3379 g ⱘ 㣃⻎䝌䫉Ammonium p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

b. ⿄ 0.4416 g ⱘ⇼ Э䝌 㣃⻎䝌Glycine p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

c. ⿄ 0.5274 g ⱘ⚳⺅䝌 㣃⻎䝌Nicotinic p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

2. ⫼Ϟ䗄 TM ⒊⎆䖯㸠⌟ DŽ⫼ϟ䴶ⱘ 䅵ㅫ ⥛DŽ 䆺㒚 䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

% ⥛ =

䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

⌟ ⱘ⌧

u 100

25

()

⹂ Ẕ 䳔ⱘ䆩 ϢҾ ˖

⇼∂ ⒊⎆ˈ⌧ Ў1000 mg/L NH3 – N ⫓⫊ TenSette ⿏⎆ 䜡 ⱘ ⏋ 䞣ㄦˈϝϾ

1. 䇏 ⌟䆩㒧 ˈ 㺙 ḋ ⱘ↨㡆ⲓ( ⠽䋼) ⬭ Ҿ ЁDŽ

ˈ ⱘḋ 䇏 ⼎ 乊ッⱘϔ㸠DŽ 䆺㒚 䇋 㾕⫼ DŽ 4. ⌧ Ў1000 mg/L NH3 – Nⱘ⇼∂ ⒊⎆DŽ 5. ⫼TenSette ⿏⎆ ϝϾ ḋDŽ ḋ ϝϾ⏋ 䞣ㄦЁˈ⎆䴶Ϣ10 mL 㒓 唤DŽՓ⫼TenSette ⿏⎆ ϝϾ⏋ 䞣ㄦЁձ⃵ 0.1 mLˈ0.2 mL 0.3 mLⱘ ⠽䋼ˈ⏋ DŽ

6. Ң0.1 mLⱘ ḋ ˈ ✻Ϟ䗄 þ TMձ⃵ ϝϾ ḋ 䖯㸠⌟䆩DŽ “Read()”䬂⹂䅸 ↣ϔϾ ḋ ⱘ⌟䆩 DŽ

7. ⌟䆩䖛⿟㒧 ˈ “Graph()”䬂 ⼎ ḍ 䅵ㅫ ⱘ Շ 㒓ˈ䇈 ⱘ Ϣ DŽ “Ideal Line()”䬂 ⼎ ḋ Ϣ100% ⥛ⱘ“⧚ 㒓 ”П䯈ⱘ ㋏DŽ↣Ͼ ḋ䛑 䆹䖒 㑺100%ⱘ ⥛DŽ

䳔ⱘ䆩 ϢҾ 䆒 ˖

⇼∂ ⒊⎆ˈ⌧ Ў10 mg/L

TM

1. ⫼2mL ⌧ Ў10 mg/Lⱘ⇼∂ ⒊⎆ҷ ḋ ✻Ϟ䗄 Test

䖯㸠⌟䆩DŽ

⒊⎆⌧ 㒓DŽ

⺅ ⱘ䖛⸿䝌Ⲥ⍜㾷䖛⿟ ⱘ∂䛑䕀 Ў⸱䝌ⲤDŽ⍜㾷㒧 ⱘ Ѯ⸿䝌⇶䩴⫼Ѣ 䰸 ㋴㉏⇻ ⠽䋼DŽ ⸱䝌ⲤϢ 㡆䝌 䝌 ⦃ ϟ ⫳ ϔ⾡咘㡆䜡 ⠽DŽ⌟䆩㒧 ⊶䭓Ў410 nmⱘ 㾕 ϟ䇏 ⱘDŽ

10072

2 – 150 mg/L N Test N Tube TM ⫼Ѣ∈Ϣ ∈DŽ

Test N Tube TM

1. DBR 200 ⍜㾷 ˈ ⛁㟇105 ćDŽ

ϸϾ2. ⫼ϔϾ ⓣ ˈ

催䞣⿟Total Nitrogen

Hydroxide ⍜㾷䆩 ㅵЁ ϔ Total Nitrogen

Persulfate ∂䖛⸿䝌Ⲥ䆩 ㉝ DŽ Ⲫ 㶎㒍Ϟ㉬ⱘ䆩 DŽ

ϔϾ䆩 ㅵ3. Ё 0.5 mLḋ DŽ

䆩 ㅵЁ 0.5 mL䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

ぎⱑ ˈ 㛑⫼ ϡ ∂ⱘ∈ҷ 䆩 㒘ӊЁ䜡 ⱘ ⾏ ∈DŽ

⣯4. ϸϾ䆩 ㅵⲪϞⲪ DŽ

⚜ 㟇 30⾦ҹ⏋ DŽ 䖛⸿䝌Ⲥ䆩 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽ

5. 䆩 ㅵ ⍜㾷 ˈⲪϞ6. ⍜㾷 䯈㒧 ˈゟ ⫼ Ⲫ DŽ ⛁⍜㾷ℷ 30 䩳DŽ ⛁ⱘ䆩 ㅵҢ⍜㾷

Ё ˈ 䆩 ㅵ Ϟ 㟇 ⏽DŽ

7. 䗝 ⌟䆩⿟ DŽ ✻ⱘ㽕∖ 䗖䜡 䙂 㔽 (䆺㒚ҟ㒡䇋 㾕⫼ ) DŽ

8. 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ Total Nitrogen (TN) A ∂A 䆩 ㉝ DŽ

Ϟϟ 䆩 ㅵ9. ⲪϞⲪ ˈ

15⾦DŽ

10. Ҿ DŽ䅵 3 䩳DŽ

11. 䅵 䯈㒧 ˈ 䆩 ㅵⱘⲪ ˈ ϸϾ䆩 ㅵЁ ϔ TN B ∂B 䆩 ㉝ DŽ

12. ⲪϞⲪ ˈϞϟ 䆩 ㅵ15⾦DŽ䆩 ㉝ 㛑ϡ㛑 ⒊㾷ˈԚ䖭ϡӮ ⌟䆩ⱘ㒧 ⱘ ⹂ DŽℸ ⒊⎆ 咘㡆DŽ

Test N Tube TM

13. Ҿ DŽ䅵 2 䩳DŽ

14. 䅵 䯈㒧 ˈ ϸϾTN C ∂C 䆩 ㅵˈ 2 mL ḋ ⍜㾷⎆ ϔϾTN C ∂C 䆩 ㅵЁˈ 2 mLぎⱑ ⍜㾷⎆ ㄀ѠϾTN C ∂C 䆩 ㅵЁDŽ

15. ⲪϞⲪ 䕀䆩 ㅵ10⃵ҹ⏋ DŽ㓧 ⱘ ⱘ 䕀䆩 ㅵDŽ

ℸ 㾺ㅵ䑿⏽ 䆹 ⱘDŽ

16. Ҿ DŽ䅵 5 䩳DŽ

ℸ ⒊⎆ⱘ咘㡆 䆹 ⏅DŽ

17. 䅵 䯈㒧 ˈ ぎⱑ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

18. ϟ“Zero()”䬂䖯㸠Ҿ 䇗䳊DŽ䖭 ⼎˖

0 mg/L N

19. 㺙 ḋ ⱘ䆩 ㅵ ˈ 16 mm 䗖䜡 ЁDŽ

ҹ䖯㸠 ϔ㒘ⱘ Ͼḋ ⱘ⌟䆩DŽ

20. ϟ“Read()”䬂䇏 ∂ 䞣ˈ㒧 ҹmg/L NЎ ԡDŽ

ℸ 䯈 ҹ⫼ℸぎⱑ ⌟ 㒘ⱘ Ͼḋ DŽ ぎⱑ 䰈 ˈ⏽ ⏽(18 – 25 ぎⱑ 䆩 䭓 ҹ 7 ˈ

ć) DŽ ϔ ˈぎ ⱑ䆩 ⦄ 䞣ⱘⱑ㡆㍂ ⠽ˈ䇋㟡 䖭Ͼ 䞡 䜡 ⱘぎ ⱑ䆩 DŽ

ϟ㸼 Ё ⼎ⱘ ⠽ ⱘ ⌧ ∈ (mg/L)ϟ ⌟䆩ϡѻ⫳ DŽ㛑 ѻ⫳f10%⌟䆩䇃 ⱘ ⠽䋼 㸼ЁDŽ

⌟䆩 ⫼ҹϟ⠽䋼䜡 ⱘ∂ ⒊⎆䖯㸠ⱘˈ 95%ⱘ ⥛DŽ g ∃ 䫉 g ㋴ g ⸿䝌䫉 g ⇼ Э䝌 g 䝟䝌

⫼∃ 䫉 ⚳⺅䝌-PTSA ⱘ ⥛ˈ ⫳⌏∵∈䖯 ∈ǃ ҷ∵∈(D5905 - 96)ⱘASTM ⠽䋼Ёⱘ ⥛䛑ı95%DŽ ѯḋ Ё ⱘ 䞣ϡ ∂ⱘ ⠽Ӯ Ў⍜㗫 ⍜㾷䆩 Ёⱘ䖛⸿䝌Ⲥ㗠䰡Ԣњ⍜㾷ⱘ ⥛DŽ ⶹḋ Ё 䕗催⌧ ⱘ䖭⾡ ⠽ˈ 䆹⿔䞞ḋ ˈ 催⍜㾷 ⥛ 䞡 䖯㸠⌟䆩DŽ

ḋ 䞛䲚 Փ⫼⏙⋕ⱘ⦏⩗ DŽ䞛ḋ ゟ ⱘ㒧 䴴DŽ

䞛ḋ ϡ㛑ゟ 䖯㸠 ⌟䆩ˈ䇋Փ⫼⸿䝌(2mL⌧⸿䝌/ L∈) ḋ ⱘpH 䇗 㟇2 㗙2ҹϟҹ DŽ ḋ 㕂Ѣ4 ć ( 39 ̧ )ⱘ ӊϟ䖯㸠 DŽḋ 䭓 ҹ 28 DŽ

⌟䆩 ˈ䇋 ḋ ⛁㟇 ⏽ˈ⫼5.0 N⇶⇻ 䩴⒊⎆Ё ḋ 䝌 ˈ ḋ ⱘpH 䇗 Ё DŽ ḍ ḋ ԧ⿃ 䞣 ℷ⌟䆩㒧 DŽ

⊩ Ѣ ∂䗮 㛑 䖒 95 ̢ 100%ⱘ ⥛DŽ ⊩⫼ ⇣∂ ⠽䋼⊩䖯㸠 ⹂ Ẕ DŽ

1. 䜡 ҹϟϝϾ⒊⎆ЁⱘϔϾ Ͼ⒊⎆DŽ↣ϔ⾡⒊⎆ⱘ⌧ 䛑Ⳍ Ѣ25 mg/L N ∂ ⒊⎆DŽ⫼䆩 㒘ӊЁⱘ ⾏ ∈ 㗙ϡ ӏԩ ⠽ ∂ⱘ∈䜡 䖭ѯ ⒊⎆DŽ

a. ⿄ 1.6208 g ⱘ 㣃⻎䝌䫉Ammonium p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

b. ⿄ 2.1179 g ⱘ⇼ Э䝌 㣃⻎䝌Glycine p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

c. ⿄ 2.5295 g ⱘ⚳⺅䝌 㣃⻎䝌Nicotinic p-Toluenesulfonate (PTSA)ˈ⫼ ⾏ ∈ ⒊㾷Ѣ1000 mLⱘ 䞣⫊Ёˈ ⫼ ⾏ ∈⿔䞞㟇1000 mL 㒓DŽ

2. ⫼Ϟ䗄 TM ⒊⎆䖯㸠⌟ DŽ⫼ϟ䴶ⱘ 䅵ㅫ ⥛DŽ 䆺㒚 䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

% ⥛ =

䇋 㾕ϟ䴶ⱘ㸼ḐDŽ

⌟ ⱘ⌧

u 100

25(120)

()

⹂ Ẕ 䳔ⱘ䆩 ϢҾ ˖

⇼∂ ⒊⎆ˈ⌧ Ў1000 mg/L NH3 – N ⫓⫊ TenSette ⿏⎆ 䜡 ⱘ ⏋ 䞣ㄦˈϝϾ

1. 䇏 ⌟䆩㒧 ˈ 㺙 ḋ ⱘ↨㡆ⲓ( ⠽䋼) ⬭ Ҿ ЁDŽ

ˈ ⱘḋ 䇏 ⼎ 乊ッⱘϔ㸠DŽ 䆺㒚 䇋 㾕⫼ DŽ 4. ⌧ Ў1000 mg/L NH3 – Nⱘ⇼∂ ⒊⎆DŽ 5. ⫼TenSette ⿏⎆ ϝϾ ḋDŽ ḋ ϝϾ⏋ 䞣ㄦЁˈ⎆䴶Ϣ25 mL 㒓 唤DŽՓ⫼TenSette ⿏⎆ ϝϾ⏋ 䞣ㄦЁձ⃵ 0.1 mLˈ0.2 mL 0.3 mLⱘ ⠽䋼ˈ⏋ DŽ

6. Ң0.1 mLⱘ ḋ ˈ ✻Ϟ䗄 Test TMձ⃵ ϝϾ ḋ 䖯㸠⌟䆩DŽ “Read()”䬂⹂䅸 ↣ϔϾ ḋ ⱘ⌟䆩 DŽ

7. ⌟䆩䖛⿟㒧 ˈ “Graph()”䬂 ⼎ ḍ 䅵ㅫ ⱘ Շ 㒓ˈ䇈 ⱘ Ϣ DŽ “Ideal Line()”䬂 ⼎ ḋ Ϣ100% ⥛ⱘ“⧚ 㒓 ”П䯈ⱘ ㋏DŽ↣Ͼ ḋ䛑 䆹䖒 㑺100%ⱘ ⥛DŽ

䳔ⱘ䆩 ϢҾ 䆒 ˖

⇼∂ ⒊⎆ˈ⌧ Ў100 mg/L

TM

1. ⫼0.5mL ⌧ Ў100 mg/Lⱘ⇼∂ ⒊⎆ҷ ḋ ✻Ϟ䗄 Test þ䖯㸠⌟䆩DŽ

⒊⎆⌧ 㒓DŽ

⺅ ⱘ䖛⸿䝌Ⲥ⍜㾷䖛⿟ ⱘ∂䛑䕀 Ў⸱䝌ⲤDŽ⍜㾷㒧 ⱘ Ѯ⸿䝌⇶䩴⫼Ѣ 䰸 ㋴㉏⇻ ⠽䋼DŽ ⸱䝌ⲤϢ 㡆䝌 䝌 ⦃ ϟ ⫳ ϔ⾡咘㡆䜡 ⠽DŽ⌟䆩㒧 ⊶䭓Ў410 nmⱘ 㾕 ϟ䇏 ⱘDŽ